Target Gene Amplification Technology
of a Clustered Genetic Variations in a Motif
TriO (Trigger Oligonucleotide) technology is a high-sensitivity, high-sensitivity PCR technology that can effectively detect target genes with various subtypes or serotypes by our specialized primer design and amplification technique.
Principle of Action
In the early reaction TriO primer is used to amplify even a small amount of minor type to generate a certain level of amplicon. Then high concentration of representative primer enables homogeneous and effective detection of various target genes.
Key Advantages
- High Inclusivity
- • TriO™ Technology can detect clustered genetic variations in a motif with minimum number of primers and is suitable for multiplex PCR
- High Specificity
- • TriO™ Technology has high sensitivity due to maximized efficiency of primer and improved primer binding.
- High Adaptability
- • TriO™ Technology can be utilized in gene amplification-based molecular diagnostics to provide highly comprehensive diagnostic results.
Application Examples
• Fluorescence intensities are improved by homogenous amplification through fusion of TriO technology to target analysis with many mutations in primer binding site, thereby improving sensitivity.
TriO PCR Ampilcon
Results of the application of the TriO on NeoPlex
