Technical Overview

• TriO (Trigger Oligonucleotide) technology is a high-sensitivity, high-sensitivity PCR technology that can effectively detect target genes with various subtypes or serotypes by our specialized primer design and amplification technique.

Principle of action

• In the early reaction TriO primer is used to amplify even a small amount of minor type to generate a certain level of amplicon.
• Then high concentration of representative primer enables homogeneous and effective detection of various target genes.

Feature point

• High Inclusivity PCR

  • TriO™ Technology can detect clustered genetic variations in a motif with minimum number of primers and is suitable for multiplex PCR

• High Sensitivity

  • TriO™ Technology has high sensitivity due to maximized efficiency of primer and improved primer binding.

• High Adaptability

  • TriO™ Technology can be utilized in gene amplification-based molecular diagnostics to provide highly comprehensive diagnostic results.

Application Examples

• Fluorescence intensities are improved by homogenous amplification through fusion of TriO technology to target analysis with many mutations in primer binding site, thereby improving sensitivity.