Technical Overview

• The Restriction Fragment Mass Polymorphism (RFMP) technology is based on DNA amplification and mass detection of oligonucleotides excised by first-generation molecular scissors, using matrix-assisted laser desorption and ionization time of flight (MALDI-TOF) mass spectrometry for clinical genotyping

Principle of action

• Multiple oligomers containing mutation sites are formed when performing PCR reaction and cutting with a molecular scissors.
• Genotypes and mutations can be identified by measuring the mass of each of these fragments.

Feature point

• High Sensitivity & High Specificity

  • Unlike most existing genotyping technologies that analyze gene variation by hybridization, RFMP technology identifies genotypes by measuring the mass of fragmented gene sequences.

• Ultra Precision & High Reproducibility

  • RFMP technology detects a minor genetic mutation and diagnose target genes with various genotypes with high sensitivity to enable early diagnosis.

Application Examples

• RFMP precisely identifies mixture of multiple mutations, whereas direct sequencing could not identify specific genotypes with a double mutation in one codon due to the inability of sequencing to determine haplotype.

* Patent No. : 10-0642829 (KR) / 4073471 (JP) / 7541148 (US) / 1113589 (HK)