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Suppression of non-specific amplification that allows control of concentration, speed, and time of specific target amplification with a unique primer structure

Technical Overview

  • EnCleaP (Enzymatically Cleavable Primer) is a high-specificity PCR technology that controls the primer activation to suppress non-specific amplification products that can occur during PCR reaction. The primer concentration, amplification rate, and timing of primer's participation in the amplification reaction can be arbitrarily controlled, maximizing the reaction specificity and sensitivity due to the focused amplification reaction of the production of specific gene products. EnCleaP technology is used in many of our platforms including NeoPlex, OmniPlex, and RFMP to improve the specificity of the diagnostic agent.

Structure and Prinicple

  • EnCleaP technology can amplify the target gene with high specificity by activating inactivated primers at specific temperatures through the GeneMatrix’s core enzyme with a unique primer structure.

Feature point

  • High Specificity

    • Primer is inactive before PCR reaction, reducing the non-specific binding. This inhibits non-specific amplification products and results in highly specific diagnostic results.
  • High Sensitivity

    • The generation of non-target products is suppressed and the specific product to be targeted is maximized for high sensitivity.
  • High Adaptability

    • EnCleaP technology can be utilized in gene amplification-based molecular diagnostics such as NeoPlex, OmniPlex, and RFMP to provide improved diagnostic results.

Application Examples

  • Multiplex PCR of intramolecular proximity target

    • Simultaneous generation of target and non-targeted products
    • Observation of non-specific reactions, reduction of amplification efficiency
  • EnCleaP PCR

    • Focused production of target products through EnCleaP Primer
  • EnCleaP Primer is activated at the end of the PCR reaction to suppress non-specific amplification products, and the specificity and sensitivity of the target product are increased.