The core technology for directly analyzing gene mutations
by DNA mass spectrometry without labeling
The Restriction Fragment Mass Polymorphism (RFMP) technology is based on DNA amplification and mass detection of oligonucleotides excised by first-generation molecular scissors, using matrix-assisted laser desorption and ionization time of flight (MALDI-TOF) mass spectrometry for clinical genotyping
Multiple oligomers containing mutation sites are formed when performing PCR reaction and cutting with a molecular scissors. Genotypes and mutations can be identified by measuring the mass of each of these fragments.
High Sensitivity & High Specificity
Unlike most existing genotyping technologies that analyze gene variation by hybridization, RFMP technology identifies genotypes by measuring the mass of fragmented gene sequences.
Ultra Precision and High Reproducibility
RFMP technology detects a minor genetic mutation and diagnose target genes with various genotypes with high sensitivity to enable early diagnosis.
RFMP precisely identifies mixture of multiple mutations, whereas direct sequencing could not identify specific genotypes with a double mutation in one codon due to the inability of sequencing to determine haplotype.