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RFMPTM Platform Technology

The core technology for directly analyzing
gene mutations by DNA mass spectrometry
without labeling

Technology Overview

The Restriction Fragment Mass Polymorphism (RFMP) technology is based on DNA amplification and mass detection of oligonucleotides excised by first-generation molecular scissors, using matrix-assisted laser desorption and ionization time of flight (MALDI-TOF) mass spectrometry for clinical genotyping

Principles

Multiple oligomers containing mutation sites are formed when performing PCR reaction and cutting with a molecular scissors. Genotypes and mutations can be identified by measuring the mass of each of these fragments.

Features

  • Detection of a minor load of HBV variants with drug resistant mutations

    Targeted therapy of hepatitis virus

    Diagnosis of drug resistance Drug-sensitive virus Drug-resistant virus

High Sensitivity & High Specificity

Unlike most existing genotyping technologies that analyze gene variation by hybridization, RFMP technology identifies genotypes by measuring the mass of fragmented gene sequences.

Ultra Precision and High Reproducibility

RFMP technology detects a minor genetic mutation and diagnose target genes with various genotypes with high sensitivity to enable early diagnosis.

Application Examples

  • Sequencing Result


    rt204
    Position 1 A or G
    Position 3 T or G

    ATG(Met)? GTG (Val)?
    GTT (Val)? ATT(Ile) ?
    Wild ? Mutant ?

    Unclear diagnostic results

  • RFMP Result


    rt204
    GTG(Val) : ATT(Ile) = 1 : 1
    Mutant : Mutant = 1 : 1

    Detection of the exact mixed genotype

RFMP precisely identifies mixture of multiple mutations, whereas direct sequencing could not identify specific genotypes with a double mutation in one codon due to the inability of sequencing to determine haplotype.

Patent No. :
10-0642829 (KR) / 4073471 (JP) / 7541148 (US) / 1113589 (HK)


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