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TriOTM Technology

Target gene amplification technology of

a clustered genetic variations in a motif

Technology Overview

TriO (Trigger Oligonucleotide) technology is a high-sensitivity, high-sensitivity PCR technology that can effectively detect target genes with various subtypes or serotypes by our specialized primer design and amplification technique.

Principles

In the early reaction TriO primer is used to amplify even a small amount of minor type to generate a certain level of amplicon. Then high concentration of representative primer enables homogeneous and effective detection of various target genes

Features

High Inclusivity PCR

TriOTM Technology can detect clustered genetic variations in a motif with minimum number of primers and is suitable for multiplex PCR

High Sensitivity

TriOTM Technology has high sensitivity due to maximized efficiency of primer and improved primer binding.

High Adaptability

TriOTM Technology can be utilized in gene amplification-based molecular diagnostics to provide highly comprehensive diagnostic results.

Application Examples

  • TriO PCR Ampilcon

Results of the application of the TriO on NeoPlex

  • (A) Before the application

  • (B) After the application of TriO

Fluorescence intensities are improved by homogenous amplification through fusion of TriO technology to target analysis with many mutations in primer binding site, thereby improving sensitivity.


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